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Journal: Science Advances
Article Title: A dual chemodrug-loaded hyaluronan nanogel for differentiation induction therapy of refractory AML via disrupting lysosomal homeostasis
doi: 10.1126/sciadv.ado3923
Figure Lengend Snippet: ( A ) Wright-Giemsa staining of cells after 5-day treatment [Cis (0.45 μg/ml) and Dau (0.175 μg/ml)]. ( B ) Immunofluorescence staining of CD14 and the statistical data of the nucleus areas stained by DAPI. Scale bar, 20 μm. ( C ) Cathepsin B evaluation by a Magic Red assay kit and the relative integrated density of Magic Red in cytoplasm. Scale bar, 10 μm. ( D ) FACS analysis of the CD14 expression with or without NAC pretreatment ( n = 3). ( E ) Relative ROS in Kasumi-1 cells incubated with Cis + Dau and HA/Cis/Dau with or without NAC pretreatment ( n = 3). ( F ) Proteome profiler array. “*,” “#,” or “&” P < 0.05; “**,” “##,” or “&&” P < 0.01; *** P < 0.001; **** P < 0.0001. Con, control.
Article Snippet: Then, the
Techniques: Staining, Immunofluorescence, Expressing, Incubation, Control